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Greater than 10mL and up to 50mL of urine is required to run the ADXBLADDER test. The patient should always be asked to provide the whole urine void, then an appropriate portion of that void can be aliquoted for use in the ADXBLADDER assay. Ensure the sample is well mixed before aliquoting.
More urine increases the potential sample available for testing.
For example:
| 50mL urine | 500μL | 4 x 100μL samples |
| 25mL urine | 250μL | 2 x 100μL samples |
| 10mL urine | 100μL | 1 x 100μL samples |
We have found that showing the patient a collection container with an example volume of 50mL of coloured water significantly increases the number of patient samples greater than 50mL.
Between 10 and 50mL of urine is required to run the ADXBLADDER test. If you have a greater volume than 50mL, this may be decanted into separate tubes and processed as additional patient samples, or simply discarded.
It is important to ensure the whole urine sample has been thoroughly mixed to prevent premature sedimentation prior to decanting into the centrifuge tubes, which could result in inconsistencies between samples.
ADXBLADDER has been developed as a manual ELISA, but like other manual ELISAs it may be validated on open automated platforms by the user. Application notes are available for some platforms – contact your Arquer Diagnostics distributor for assistance. Each lab should validate ADXBLADDER on their system.
Arquer Diagnostics test samples and controls in duplicate and take a mean of the reading in order to control for pipetting errors.
Arquer Diagnostics have tested the assay for sensitivity to a range of contaminants that can commonly be found in some urine samples such as blood or cellular components. See the product “Instructions for Use” document “12.3 Potentially Interfering Substances” for details.
Urine must be processed within 48 hours of collection.
Samples may be stored at room temperature for up to 8 hours and then at 2 – 8°C for up to 48 hours prior to processing. Do not freeze urine before processing.
